Device

Part:BBa_M11205

Designed by: Angela Dixon   Group: UtahState BE5930 - S09 UtahState BE5930 - S10   (2010-04-22)


HXT1 promoter + GFP to monitor glucose levels in S. cerevisiae

Used in S. cerevisiae to monitor glucose levels. The promoter, HXT1, is activated in the presence of glucose. The promoter is followed by the Kozak sequence Ribosome Binding Site. After the RBS, is the GFP sequence codon optimized for S. cerevisiae. It is ended with the ADH1 terminator for S. cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2389


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Categories
Parameters
None